Flow cytometry in allergy diagnosis – basophil identification protocols

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Published: Dec 12, 2012
Keywords:
allergy, diagnostic, flow cytometry, basophil

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Anna Skotny
Katedra i Klinika Chorób Wewnętrznych, Geriatrii i Alergologii Uniwersytetu Medycznego im. Piastów Śląskich we Wrocławiu
Barbara Kmiecik
Zakład Chirurgii Eksperymentalnej i Badania Biomateriałów Uniwersytetu Medycznego im. Piastów Śląskich we Wrocławiu
Ewa Zbrojewicz
Katedra i Klinika Chorób Wewnętrznych, Geriatrii i Alergologii Uniwersytetu Medycznego im. Piastów Śląskich we Wrocławiu
Emilia Siwak
Katedra i Klinika Chorób Wewnętrznych, Geriatrii i Alergologii Uniwersytetu Medycznego im. Piastów Śląskich we Wrocławiu
Wojciech Mędrala
1. Katedra i Klinika Chorób Wewnętrznych, Geriatrii i Alergologii Uniwersytetu Medycznego im. Piastów Śląskich we Wrocławiu. 2. Zakład Badań Klinicznych, Wyższa Szkoła Medyczna w Legnicy

Abstract

Diagnosis and treatment of allergic diseases are a very up to date topics. The modernization and improvement of existing diagnostic methods are essential for the progress of medicine. Flow cytometry fits into this trend. It should be noted that this method allows one to study cell populations constituting less than 1% of the sample size. During flow cytometry diagnostics, several parameters can be identified and analyzed at the same time. There are few advantages (particularly in the case of safety) for patients in in vitro allergy diagnosis. There is no possibility to cause anaphylaxis or other dangerous complications as in in vivo food, drug or insect venom allergy diagnosis. The article discusses the advantages of flow cytometry in the context of using it in the diagnosis of allergic diseases. Problems associated with basophils identification and types of protocols used in basophils activation tests (BAT) are widely discussed.

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How to Cite
Skotny , A., Kmiecik , B., Zbrojewicz , E., Siwak , E., & Mędrala , W. (2012). Flow cytometry in allergy diagnosis – basophil identification protocols. Alergoprofil, 8(4), 14-18. Retrieved from https://journalsmededu.pl/index.php/alergoprofil/article/view/747
Issue
Vol 8 No 4 (2012)
Section
Article

Copyright: © Medical Education sp. z o.o. This is an Open Access article distributed under the terms of the Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). License (https://creativecommons.org/licenses/by-nc/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.

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