Evaluation of expression of CD203c on the activated basophil surface in diagnosis of selected allergic disorders
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Abstract
Introduction: Increased incidence of the allergic-related diseases shows the significance of proper diagnosis. The methods being employed nowadays seem to be ineffective in some cases and considerably restricted. These procedures happen not to be practiced because they either do not comply with the requirements or there is danger of high risk life threatening anaphylactic reaction.
Objective: The aim of this research is estimate of expression CD203c in diagnosis of allergy to selected allergens: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Timothy pollen and comparison these results with total and specific IgE tests.
Material and methods: The studies was carried out in a group 40 patients and in 29 healthy control. Skin prick test was performed with aeroallergens and total and specific IgE concentration was measured. To analyse basophils marked with the one anty-CD203c or cocktail of three antibodies (anty-CRTH2, anty-CD3 and anty-CD203c) the cytometric method has been used in this research. Results: Sensitivity of cytometric method was differentiated and depended on allergen (from 43.5% for Dermatophagoides pteronyssinus to 72.4% for Timothy pollen). Joined specific IgE with the cytometric method achieved findings enabled to increase the sensitivity to 78.3% for Dermatophagoides pteronyssinus and to 89.7% for Timothy pollen. In these assays specificity of cytometric method increases up to 100%.
Conclusions: Evaluation of CD203c expression is useful in aeroallergens allergy testing especially at person for whom another diagnostic methods do not comply with the requirements or there is danger of high risk life threating anaphylactic reaction.
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Copyright: © Medical Education sp. z o.o. This is an Open Access article distributed under the terms of the Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). License (https://creativecommons.org/licenses/by-nc/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
Address reprint requests to: Medical Education, Marcin Kuźma (marcin.kuzma@mededu.pl)
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